Automated unit test generation using large language models (LLMs) holds great promise but often struggles with generating tests that are both correct and maintainable in real-world projects. This paper presents KTester, a novel framework that integrates project-specific knowledge and testing domain knowledge to enhance LLM-based test generation. Our approach first extracts project structure and usage knowledge through static analysis, which provides rich context for the model. It then employs a testing-domain-knowledge-guided separation of test case design and test method generation, combined with a multi-perspective prompting strategy that guides the LLM to consider diverse testing heuristics. The generated tests follow structured templates, improving clarity and maintainability. We evaluate KTester on multiple open-source projects, comparing it against state-of-the-art LLM-based baselines using automatic correctness and coverage metrics, as well as a human study assessing readability and maintainability. Results demonstrate that KTester significantly outperforms existing methods across all metrics, producing tests with clearer intent and higher practical value.