Large Language Models (LLMs) have revolutionized human-AI interaction by enabling intuitive task execution through natural language prompts. Despite their potential, designing effective prompts remains a significant challenge, as small variations in structure or wording can result in substantial differences in output. To address these challenges, LLM-powered applications (LLMapps) rely on prompt templates to simplify interactions, enhance usability, and support specialized tasks such as document analysis, creative content generation, and code synthesis. However, current practices heavily depend on individual expertise and iterative trial-and-error processes, underscoring the need for systematic methods to optimize prompt template design in LLMapps. This paper presents a comprehensive analysis of prompt templates in practical LLMapps. We construct a dataset of real-world templates from open-source LLMapps, including those from leading companies like Uber and Microsoft. Through a combination of LLM-driven analysis and human review, we categorize template components and placeholders, analyze their distributions, and identify frequent co-occurrence patterns. Additionally, we evaluate the impact of identified patterns on LLMs’ instruction-following performance through sample testing. Our findings provide practical insights on prompt template design for developers, supporting the broader adoption and optimization of LLMapps in industrial settings.