In large-scale enterprises, on-call engineers (OCEs) are critical for ensuring service availability and reliability. However, as incidents grow in volume and complexity, traditional manual on-call processes are becoming increasingly inadequate. Recent advances in large language models (LLMs) have demonstrated remarkable capabilities in reasoning and multi-agent collaboration, presenting new opportunities for automation. We propose OncallX, an end-to-end automated on-call system designed for real-world industrial scenarios that integrates LLMs with multi-agent cooperation to enable intelligent and efficient incident management. OncallX first enhances user queries by leveraging external knowledge bases and multi-turn dialogue interactions. Subsequently, multiple expert agents collaborate through tree-search-based mechanisms to generate effective responses and solutions. When incidents cannot be resolved automatically, OncallX accurately assigns them to the most appropriate teams. Comprehensive experiments conducted in the real-world production environment of a top-tier global online video service provider demonstrate that OncallX efficiently responds to incidents and accurately triages tickets, significantly outperforming existing methods in both automated metrics and human evaluations. Furthermore, OncallX has been successfully deployed in production for two months, during which it has substantially enhanced on-call efficiency, reducing average incident response time to just 21 seconds and average triage time to 4 seconds—representing a transformative improvement in operational excellence.